Pan African Journal of Life Sciences(PAJOLS)

A publication of Faculty of Basic Medical Sciences and Faculty of Basic Clinical Sciences,
Ladoke Akintola University of Technology, Ogbomoso

e-ISSN: 2672-5924
Volume 4, No. 3, December 2020
Pages 121-126

DOI: 10.36108/pajols/0202/40(0330)

Evidence of Metabolic Resistance in Pyrethroid Resistant Aedes aegypti Population from Lagos, Nigeria

Kemi O. Adesalu1, Adedapo O. Adeogun1, Tolulope A. Oyeniyi1, Abiodun K. Olakiigbe2, Romoke T. Jimoh1, Ifeoluwa K. Fagbohun3, Ahmed I. Omotayo1, Lekan Olagundoye1, Samson T. Awolola1
1Department of Public Health and Epidemiology, Nigerian Institute of Medical Research, Lagos, Nigeria
2Grant, Monitoring and Evaluation Unit, Nigerian Institute of Medical Research, Lagos, Nigeria
3Department of Zoology, University of Lagos, Nigeria


Background: Pyrethroid resistance in Aedes aegypti is of major concern to the control of several arboviral infections. The major mechanisms of Pyrethroid resistance in mosquitoes are target-site insensitivity and elevation in the activity of detoxification enzymes. In this study, we assessed the susceptibility status of Aedes aegypti population from Lagos to Pyrethroid and the impact of metabolic enzymes on resistance development.
Methods: Larvae of Aedes aegypti were collected from differ ent habitats in Lagos Mainland Local Government Area, Lagos state. Adult mosquitoes of 2-5 days were exposed to diagnostic dose of permethrin and Deltamethrin using the CDC method. Synergist assay was done with pre-exposure of samples to PBO before exposure to insecticide. Esterase and GST activities were measured using standard protocol. Regression Probit was used to compute the KDT50 and KDT95. Analysis of variance was used to compare the difference in mean of enzymes activities.
Results: Aedes aegypti population from the study location is resistant to permethrin (33% ) and Deltamethrin (80%) within the diagnostic time. PBO pre-exposure increases percentage knockdown from 33% to 82% and 80% to 87% for permethrin and Deltamethrin respectively. The activity of GST was higher (P<0.05) in permethrin exposed mosquitoes in comparison with unexposed.
Conclusion: A robust insecticide resistance management (IRM) plan in Lagos should take into consideration strategies for addressing the effects of metabolic enzymes in resistance development .
Keywords: Metabolic, Resistance, Pyrethroid, Resistant, Aedes aegypti


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